Development of a tick-borne pathogen real-time PCR panel for the detection of Anaplasma, Ehrlichia, and Borrelia spp. Ji-In Hum & Benjamin Hon
team: Ji-In Hum, Benjamin Hon, Min-Kuang Lee, Navdeep Chahil, Muhammad Morshed
To enhance the current surveillance in tick borne disease in British Columbia, we aim to expand our existing Borrelia burgdorferi 23S Taqman PCR by integrating two additional tick-borne pathogens, Ehrlichia and Anaplasma, into the assay. This triplex real-time PCR test was designed to cover most of the species under these three genera which serves as a Pan-detection of tick borne pathogen screening panel. The real-time PCR assay described in this talk is found to be a reliable method for screening of Borrelia, Anaplasma and Ehrlichia species. Using retrospective samples, we were able to detect the newly identified Candidatus Ehrlichia khabarensis , which was identified using next generation sequencing in the past. Implementation of this triplex assay will enhance vector surveillance program in BC.
BC CDC Presenters
10/16/2020 10:15:00 PM
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